Volumetric Tissue Imaging
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High Resolution – down to 20 µm
Research Advantages
From Image to Insight
Further Insights from Block Face Imaging
How It Works

Xerra™: EMIT Imaging's Flagship Platform

XerraTM, is EMIT Imaging’s flagship preclinical imaging platform available for purchase. Xerra is designed to advance discoveries in biological and drug research and fully automates the CFT workflow, providing 3D images with high resolution and sensitivity. This enables researchers to easily run samples in-house, and obtain unmatched visualization of drug distribution and protein expression in whole-animals. See what you’re missing compared to standard 2D in vivo fluorescence imaging techniques and utilize CFT to discover more. 


Xerra provides:

  • Automated CFT workflow
  • Anatomical, RGB white-light images registered with fluorescence images 
  • Capable of Multiplexing
  • 5 magnifications: 20-55 µm pixel resolution
  • 6 excitation lasers: 470 to 780 nm
  • 7 emission filters: 500 to 850 nm

How CFT Works

Step 1: Preparation

Cryo-preserve & embed the sample in OCT

Step 2: Image + Section

High resolution, high sensitivity white light and fluorescence images are acquired. The block is sectioned and 20-55 microns are removed from the block surface. Process is repeated until the whole sample is sectioned and imaged.

Step 3: Output

3D image stacks are generated. White light and fluorescence data can be overlaid.

Research Advantages

High Resolution:

High, near-cellular resolution down to 20 µm

High Sensitivity:

Nanomolar sensitivity, comparable to nuclear medicine

High Throughput:

Several samples in the same block = large volume of tissue imaged


Fits into any experiment, complementing tissue and radiology imaging


No fixation, perfusion, clearing or radiolabeling required


6 lasers and 7 filters for multiplexed applications



High Resolution

20 µm to 55 µm isotropic voxels depending on FOV

True 3D

Reconstruct whole body & large tissue data from white light and fluorescence images


Leverage built-in, turn-key workflows for image acquisition

  • Obtain automated reconstruction of 2D slices into 3D
  • Anatomical, RGB white-light images registered with high sensitivity fluorescence imaging, capable of multiplexing


Sample preparation is simple and does not require fixation, perfusion, clearing, or radiolabeling

  • Sac to image in as little as 4 hours. Frozen samples can be stored for weeks before imaging as long as they are not blocked in OCT
  • Xerra is compatible with most existing fluorophores
  • Xerra requires no infrastructure changes for install


Xerra has 6 excitation lasers ranging from 470 to 780 nm and 7 emission filters ranging from 500 to 850 nm


CFT has advantages for quantification and EMIT Imaging fully characterizes fluorophores and optimizes laser power exposure time, attenuating conditions, and subsurface signal analysis to improve quantification

  • Signal is surface-weighted
  • Signal is linear

6 Excitation Lasers

470-780 nm

7 Emission Filters

500-850 nm

Record Multiple Exposures

Recording at variable exposure times (5, 50, 500, 1500, 25000 milliseconds)

Optical Field of View

Max: 24 cm x 14 cm
Min: 8 cm x 5 cm

Working Volume(s)

FOV A: Pixel – 20 µm, Block Size – 8x6x4 cm, Sample – Tissue

FOV B: Pixel – 3µm, Block Size – 10x8x5 cm, Sample – 1 Mouse

FOV C: Pixel – 35 µm, Block Size – 14x11x6 cm, Sample – 3 Mice

FOV D: Pixel – 45 µm, Block Size – 18x14x8 cm, Sample – 4 Mice

FOV E: Pixel – 55 µm, Block Size – 24x14x10 cm, Sample – 5 Mice or 1 Rat

Section Thickness

20 μm to 50 μm


Touch Screen Display

Quality Analysis (QA)

Real-time Slice-by-slice QA

Manual Section Collection


Chamber Temperature

Refrigerated chamber, -20°. Auto defrost functionality

Image Analysis and Quantification

Compatible with Various Software Packages

Data Management

WIFI connectivity or USB A and C data ports

Discover More with Cryo-Fluorescence Tomography