Xerra: for high sensitivity detection of nanomaterials
Nanotechnology is rapidly emerging in the fields of preclinical imaging, and its uses span many applications. CFT is useful in the development and evaluation of nanomaterials for tissue imaging and drug development. Nanomaterials coupled with a fluorescent reporter group, allow for visualization of these processes with CFT. Examples include nanoadjuvants with immunomodulatory properties; the nano-knife, an almost non-invasive method of destroying cancer cells with high voltage electricity; and carbon nanotubes, a popular way of repairing damaged tissues.
Case study: Nanoemulsion imaging in mouse tumor model
Study details: Mice bearing xenografts were administered V-Sense, a perfluorocarbon nanoemulsion containing fluorine-19 (19F) and a NIR fluorophore. When administered intravenously (IV), V-Sense is preferentially taken up by cells of the reticuloendothelial system, including Kupffer cells and macrophages, especially in inflamed tissues (e.g. tumors), thus enabling imaging using both MRI and fluorescence imaging. Immediately following MRI, animals underwent FLI and were then frozen for CFT. V-Sense signal was detected in both liver and tumor, in agreement with IVIS and MRI, but also detected signal in bone marrow and lymph nodes.
Results: When compared to MRI, CFT was more sensitive in detecting baseline V-Sense signal and provided higher resolution.
Multi-spectral macrophage imaging in xenograft mice
Mice bearing xenografts were administered V-Sense and imaged in three modalities.
Study Design
Mice bearing xenografts were administered V-Sense and imaged in three modalities.
- - V-SENSE: A perfluorocarbon nanoemulstion
LOW RESOLUTION
LOW SENSITIVITY
CFT
HIGH RESOLUTION and SENSITIVITY
Normalized Corrected Counts
0.35
0.001
Hemi Dimant, et al. Macrophage tracking using multi-modality 3D imaging in xenografts. AACR 2020.
Study Design
Mice bearing xenografts were administered V-Sense and imaged in three modalities.
- - V-SENSE: A perfluorocarbon nanoemulstion
LOW RESOLUTION
LOW SENSITIVITY
CFT
HIGH RESOLUTION and SENSITIVITY
Normalized Corrected Counts
0.35
0.001
