When incorporating CFT into longitudinal studies, we can visualize and compare biodistribution of a test article over various time points. Data can be easily segmented in up to 108 regions using the 3D brain atlas tool. Â Data can be represented as an image time course or as a time course plot.
A fluorescent test article was administered to 6 rodents. At various time points neuro tissue was collected and analyzed on our mice Cryo-Fluorescence Tomography platform. After tissue sectioning was completed images were reconstructed in to 3D volumes. Using VivoQuant’s brain atlas tool 13 regions of interested were generated for the 6 time points.
The CFT platform allows for real time visual feedback during tissue sectioning. In this study the operator was able stop the process and collect a tissue section for down stream Â processing. Tissue can be collected on glass or on tape. The collection process is dependent on the downstream application as well as the size of the tissue section.